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        Pyrene phosphoramidite dU

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        產品名稱: Pyrene phosphoramidite dU
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        簡單介紹

        訂購貨號 產品名稱及規格 價格(¥)11460 Pyrene phosphoramidite dU, 100 mg 1950.0021460 Pyrene phosphoramidite dU, 250 mg 4160.0041460 Pyrene phosphoramidite dU, 1 g 11440.00


        Pyrene phosphoramidite dU  的詳細介紹
        abcam sigma roche qiagen merck millipore
        invitrogen life ambion illumina thermo ABI
        enzo vectorlabs chemicon Quidel peprotech cayman
        Biovision chondrex Meridian Kamiya Genetex Neuromics

        訂購貨號 產品名稱及規格 價格(¥)
        11460 Pyrene phosphoramidite dU, 100 mg 1950.00
        21460 Pyrene phosphoramidite dU, 250 mg 4160.00
        41460 Pyrene phosphoramidite dU, 1 g 11440.00


        Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.
        With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5', internal, or 3' position (using universal support).
        This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.


        General properties

        Appearance:

        yellowish foam

        Molecular weight:

        955.04

        Molecular formula:

        C57H55N4O8P

        Solubility:

        good in dichloromethane and acetonitrile

        Quality control:

        NMR 1H (95%) and 13C, TLC, HPLC

        Storage conditions:

        Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

        MSDS:

        Download

        Spectral properties

        Excitation maximum, nm:

        260; 282; 365; 392

        Extinction coefficient at excitation maximum, Lmol-1cm-1:

        12600; 21900; 16000; 14200

        Emission maximum, nm:

        460

        Fluorescence quantum yield:

        ~0.1

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