dsGreen Gel Staining Solution, 10000×
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產品名稱: dsGreen Gel Staining Solution, 10000×
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訂購貨號 產品名稱及規格 價格（￥）20010 dsGreen Gel Staining Solution, 10000×, 1 mL 2340.00
dsGreen Gel Staining Solution, 10000×
的詳細介紹
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abcam
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sigma
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roche
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qiagen
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merck
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millipore
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invitrogen
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life
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ambion
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illumina
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thermo
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ABI
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enzo
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vectorlabs
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chemicon
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Quidel
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peprotech
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cayman
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Biovision
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chondrex
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Meridian
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Kamiya
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Genetex
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Neuromics
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訂購貨號
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產品名稱及規格
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價格（￥）
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20010
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dsGreen Gel Staining Solution, 10000×, 1 mL
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2340.00
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SYBR Green I是一種敏感的dsDNA結合染料，可以用于日常瓊脂糖和聚丙烯酰胺凝膠的DNA檢測。
不同于Ethidium，SYBR Green I對雙鏈DNA有高度選擇性，更低的毒性，更高的靈敏度。
溴化乙錠（Ethidium Bromide）和SYBR Green I的對比
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Feature
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Ethidium Bromide
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SYBR Green I
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Fluorescence
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Red (615 nm)
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Green (524 nm)
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Excitation maximum
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302 nm
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454 nm
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Excitation light source
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UV only
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Blue light or UV
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Sensitivity
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2 ng / band(dsDNA)
100 ng / band (RNA)
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0.08 ng / band (dsDNA)
1-2 ng / band (oligonucleotides)
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Health hazard
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High
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Low
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推薦手冊：
SYBR Green I DNA凝膠染色
Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
General properties
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Appearance:
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orange solution
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Quality control:
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NMR 1H (95%) and 13C, TLC, functional testing
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Storage conditions:
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Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
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MSDS:
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Download
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Spectral properties
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Excitation maximum, nm:
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454
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Extinction coefficient at excitation maximum, Lmol-1cm-1:
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73000
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Emission maximum, nm:
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524
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Fluorescence quantum yield:
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0.8
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